Interactions of extractable nuclear antigen (ENA) and double stranded DNA

Detection of antibodies to double stranded DNA and extractable nuclear antigen.

349 Detection of Anti-nucclear Antibodies (ana) Used for Diagnostic Approach of Systemic Autoimmune Diseases. Correlation with Double Stranded DNA (DSDNA) and Extractable Nuclear Antigen (ENA) Antibodies

revealed that the levels of IgG-abs to HSV were being increased in proportion to a severity of AD. Maximal increased level of IgG-abs to HSV was in children with severe AD (Me 1⁄4 2.93 units of OD (0.14–3.15); P , 0.05). Conclusions: The detection of the elevated levels of the autoantibodies, especially against the background of herpetic infection, amplifies the phenotypic and diagnostic criter...

Proliferating cell nuclear antigen loaded onto double-stranded DNA: dynamics, minor groove interactions and functional implications

Proliferating cell nuclear antigen (PCNA) acts as a biologically essential processivity factor that encircles DNA and provides binding sites for polymerase, flap endonuclease-1 (FEN-1) and ligase during DNA replication and repair. We have computationally characterized the interactions of human and Archaeoglobus fulgidus PCNA trimer with double-stranded DNA (ds DNA) using multi-nanosecond classi...

Extractable nuclear antigen effect on the DNA anti-DNA reaction and NZB/NZW mouse nephritis.

Extractable nuclear antigen (ENA) is composed of at least two components, one a ribonucleo-protein sensitive to ribonuclease or heat and the other a protein. Antibodies to ENA are associated with a relatively benign clinical course in patients with systemic lupus erythematosus (SLE) in which DNA anti DNA complexes are thought pathogenic. The effect of ENA and anti-ENA on DNA anti-DNA reactions ...

Bending and Base-Stacking Interactions in Double-Stranded DNA

An elastic model for double-stranded polymers is constructed to study the recently observed DNA entropic elasticity, cooperative extensibility, and supercoiling property. With the introduction of a new structural parameter (the folding angle w), bending deformations of sugar-phosphate backbones, steric effects of nucleotide base pairs, and base-stacking interactions are considered. The comprehe...